Onder andere met: Hugh Jackman (Tomas/Tommy/Dr. Tom Creo), Rachel Weisz (Queen Isabel/Izzi Creo), Ellen Burstyn (Dr. Lillian Guzetti), Mark Margolis (Father Avila), Stephen McHattie (Grand Inquisitor Silecio), Fernando Hernandez (Lord of Xibalba), Cliff Curtis (Captain Ariel), Sean Patrick Thomas (Antonio), Donna Murphy (Betty), Ethan Suplee (Manny), Richard McMillan (Henry), Lorne Brass (Dr. Alan Lipper), Abraham Aronofsky (Lab Technician), Renee Asofsky (Lab Technician), Anish Majumdar (Dr. Spencer)